FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516080
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC17650.036.3344651
TCGCTCT18550.032.603584
TCTGTCG15950.032.0467768
CGCTCTG20400.029.6464615
TCTCGCT21250.028.1594312
CTCGCTC21000.027.428413
CTGTCGC20500.025.9875019
GTCTTGC33650.024.4722671
GTCTTAC13800.023.2356281
GGTATAC7250.023.1190851
GTATAGG8250.022.966791
GTATTAG12950.022.509771
GTGTAAA21400.022.1350461
GTGTTAG26600.021.9174061
TAATACT10500.021.2570174
GACGTAA2404.1964697E-521.2553181
GTATTAC8650.021.0622481
GTATTAT9750.020.9283141
GTATAGA9800.020.8215391
GTGTTAC15150.020.6841031
Produced by FastQC (version 0.11.5)