FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3391508
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13800.038.0805938
GTCTCGC16950.030.9929281
GTCTTGC31100.030.9680181
GTCTTAC12750.030.9017751
TCGCTCT18000.030.7948554
CTGTCGC18850.027.496739
TCTCGCT19700.026.3912532
CTCGCTC20250.026.387633
GTATTAA10000.026.2665081
GTATTAC7350.024.8171861
GTACTAG5800.023.9015181
TTCACCG10550.023.8822655
GTATAAT8900.023.7743051
GTATTAT8650.023.6179281
GTACTAA7000.022.931081
GTGTTAC14650.022.9097371
ACGGTAC2552.8064478E-622.6539293
GTACATA11000.022.5520531
TTGCTCT43700.022.403584
GTCTCAC39450.022.1939241
Produced by FastQC (version 0.11.5)