FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733646
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14150.042.735278
GTCTCGC16750.036.174321
TCGCTCT17750.035.2845464
CTGTCGC19350.034.5991679
GTGTTAG25600.031.6533831
GTCTTGC33150.031.2710131
TCTCGCT20400.029.0326212
CGCTCTG20800.027.6879165
GTATTAC8600.027.1636831
CTCGCTC21200.026.9149653
GTGTAAC10050.026.8765051
GGTATAC7250.026.180171
GTCTCAC46250.024.7813551
GTATTAA11050.024.4442421
GTGTAAA20250.023.7933751
GTCTTAC14750.023.7567121
GTATAGA9100.023.26451
TTGCTCT46050.022.3547614
GTATAAT8500.022.3301471
GTGTAGG20300.022.2962971
Produced by FastQC (version 0.11.5)