FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3733646
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16250.036.7822951
TCTGTCG16700.034.91868
TCGCTCT17750.031.2310414
GTCTTGC34100.030.5675071
GTATAAC6250.029.1566981
CTCGCTC19300.027.97683
TCTCGCT21000.026.7745572
GTCTTAC14250.026.0875741
GTGTTAC15200.025.8957541
GTGTAAA18400.025.7498021
CTGTCGC23250.025.3909639
GTATTAT9850.025.1606031
GTACTAT7600.024.9366511
GTATAAT8850.024.7090641
GTGTTAG25350.024.441021
CGCTCTG22150.024.052055
GTATAAG9850.023.6805671
GTATTAA9650.023.4160021
GTGTAAC12500.023.325361
GTTAGCC21450.023.158793
Produced by FastQC (version 0.11.5)