FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3540987
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC17100.036.7019961
TCTGTCG14950.033.7067388
TCGCTCT18700.031.1819524
TCTCGCT20100.030.1821172
GTCTTGC32800.030.0363541
GTATAGA9450.028.5730921
GTGTATA17350.028.1814041
CTGTCGC19800.026.1774439
CTCGCTC22450.025.7359473
CGCTCTG22500.025.595725
GTATAAT8950.024.4616371
GTCTCAC42000.023.6307181
GTGTAGG20950.022.6420971
TACACTG21950.022.6294735
GTGTTAG22700.022.5039881
GTATTAC8100.021.6228791
GCGTAGT3406.011214E-821.4638881
GTATTGC9600.021.285021
GTATAAA12700.021.261081
GTATCGG1750.003523979320.8506341
Produced by FastQC (version 0.11.5)