FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3400700
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16000.041.0610161
TCTGTCG14950.037.5580948
TCGCTCT17600.032.721064
TCTCGCT18800.032.2684862
GTATTAC6750.030.2803861
CTGTCGC19900.030.0244549
GTCTTGC31700.029.9358251
CTCGCTC19800.028.450283
CGCTCTG20100.028.293145
GTATTAG11050.026.4243851
GTCTTAC13150.026.0903111
GTGTAAT14350.025.434621
GTGTTAC14600.024.4991151
GTATAGT8350.024.4781591
TACACCG3251.6170816E-924.3646075
TCTGTCA41450.023.4454928
GTCTCAC41550.023.1904961
GTATAAG9300.022.7626191
GTATCGA1902.302913E-422.7325296
GTGTAGA17250.022.4281751
Produced by FastQC (version 0.11.5)