FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3400700
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14850.040.2459641
TCTGTCG13850.040.0243158
TCGCTCT16000.032.8501134
CTGTCGC18800.030.251879
TCTCGCT19100.030.1935842
GTATAGG9900.030.1844751
GTCTTGC31900.029.930611
CTCGCTC18450.026.9269283
CGCTCTG20750.025.6768235
GTGTTAC14600.023.9620171
GTATTAG10950.023.2964041
GTCTCAC40500.023.2150521
TACACTG20100.022.9251175
GTTTCAC49500.022.690993
GGGTTTC54900.022.568961
GTGTAGG21850.022.3490121
GTGTAAA18650.022.275681
GTGTTAG21300.022.241741
GTGTAAG16450.022.1533341
TTGCTCT42800.022.0374624
Produced by FastQC (version 0.11.5)