FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3463178
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15100.043.3824428
GTCTCGC16000.041.4915051
TCGCTCT16900.039.187784
GTCTTGC34100.034.4436571
CTGTCGC21000.032.2224169
TCTCGCT19500.031.8567852
CGCTCTG19650.031.1390915
CTCGCTC20500.029.950443
GTATAGA8450.029.35351
GTGTTAG23800.026.9738831
GTATACG2601.07951564E-725.2526451
GTCTTAC13350.025.137051
GTATATA13650.024.5845871
GTTAGCC19200.023.7015883
GGTATAC7100.023.6323681
TTGCTCT44200.023.4525344
GTATTAC7850.023.2331491
GTGTAAA19550.023.13571
GTATTAT7950.022.940911
GTGTAAT14000.022.92781
Produced by FastQC (version 0.11.5)