FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4009480
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17300.044.5247158
CTGTCGC23400.034.7637069
GTCTCGC18350.034.6050831
GTCTTGC39650.031.478181
TCGCTCT20350.029.0076754
GTCTTAC15850.026.7088641
TCTCGCT23000.026.0600512
CTCGCTC23950.025.6293983
GTATTAA11600.024.5393641
GTATAAC7500.024.3296261
GTGTTAC16900.024.1856631
GTATTAT12750.024.0433961
CGCTCTG24300.023.9961855
GTCTCAC47500.023.9710851
GTGTAAC12050.023.6229551
GTATTAG13500.023.248311
GGTATAC7850.023.2448651
GTGTAGA19650.022.6581271
GTATAAT11400.022.4088691
GTACTAC8150.021.493661
Produced by FastQC (version 0.11.5)