FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4009480
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16850.041.01648
GTCTCGC17000.040.2985271
TCGCTCT18450.033.9526824
CTGTCGC23600.030.5052229
GTCTTGC39200.029.3752291
TCTCGCT20750.029.1784482
GTCTTAC14600.025.9573691
CTCGCTC22900.025.7827683
CGCTCTG23400.025.5392445
TCCGTAC3701.0913936E-1125.2983863
GTCTCAC46900.024.552431
GTATAGG10550.024.1783051
GGTATAC9000.023.4836561
GTATAGT10100.023.0907941
GTATATA17500.022.9052411
GTATTAT11150.022.8772281
GTATAGC9600.022.7750971
GTACGAC1902.2993858E-422.737823
TTGCTCT50150.022.6849254
GTATAGA12550.022.6480661
Produced by FastQC (version 0.11.5)