FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4604592
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18500.038.522718
GTCTCGC21300.033.6216241
GTCTTGC43050.032.7609441
TCGCTCT23700.030.6779544
CTGTCGC26150.029.4554339
TCTCGCT25500.027.4201642
GTATAAG12650.026.5729371
GTCGTAC1709.780774E-525.7913821
CTCGCTC26350.025.7149523
GTGTATA23750.024.3072471
CGCTCTG27550.024.3004725
GTCTTAC18900.023.9718841
TTGCTCT59100.023.7520164
GTATAGA13000.023.0469151
GTGTAAG22350.022.8872051
GTATAGC9700.021.8473381
CTTGCTC58600.021.6497823
GTGTTAG33900.021.1250931
GTTTCAC75600.021.0721573
GTGTAGC20200.020.9820981
Produced by FastQC (version 0.11.5)