FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4167244
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18150.039.2666858
GTCTCGC18950.036.5979351
TCGCTCT21450.032.5548254
GTCTTGC42800.031.3845731
CTGTCGC23100.030.5407549
TCTCGCT24500.027.6587372
CTCGCTC24600.027.2532583
CGCTCTG25300.027.0317385
TTCACCG11700.025.842495
GTATAAC7200.025.348351
ACCGTGT14050.024.5940828
GTATTAC10750.024.44761
GTCTCAC55150.024.2241061
GTGTATA22600.023.5806941
GGGTTTC72050.023.3042981
GGTATAC8500.023.1892661
TTGCTCT56150.022.9495494
GTGTTAC18800.022.5222781
GTGTTAG27250.022.2358511
GTATTAG13200.022.1221981
Produced by FastQC (version 0.11.5)