FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4167244
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16800.038.5677578
GTCTTGC39800.035.5164071
GTCTCGC18600.034.2579271
CTGTCGC23150.032.0315069
TCGCTCT21700.027.2038024
ACGGTCG1358.427045E-426.6631685
TCTCGCT24200.025.6070792
TTGCTCT53700.025.3374564
GGGTTTC73200.023.7133031
GTATTAA12800.023.4600351
TCGACCG1550.001884815923.2235939
GTGTAAT17050.023.196591
CTCGCTC24400.023.0133723
GTCTTAC17600.022.887841
GTTTCAC67500.022.5037123
CTTGCTC55800.022.4486683
CGCTCTG25600.021.934625
TAATACT12700.021.5404824
GTATTAG14050.021.3728451
GTGTTAG29950.021.2753731
Produced by FastQC (version 0.11.5)