FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4831942
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG21300.036.1658868
GTCTCGC22600.033.3693581
TCGCTCT22650.030.513284
GTCTTGC45950.030.1158621
CTGTCGC28450.029.3542259
CTCGCTC26800.026.3255333
GTATTAG15200.025.530031
GTTTCAC70250.024.5952243
CGCTCTG26700.023.9974235
GGGTTTC78250.023.7666741
TCTCGCT29750.023.4915872
GTCTCAC54100.023.2782441
GTATTAC12050.023.0895581
GTGTTAG34000.022.8268491
GGTATAC9650.022.7621361
GTCTTAC18150.022.590741
TTGCTCT63100.022.36214
GTTAGCC24800.022.062153
GTATACG3004.149315E-721.9654581
GTACTAC8600.021.2843591
Produced by FastQC (version 0.11.5)