FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4592119
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17800.036.398368
GTCTCGC19850.036.0644451
GTCTTGC46000.032.7132071
TCGCTCT22750.032.592314
CTGTCGC22850.030.2443569
TCTCGCT24900.028.3730472
CTCGCTC26900.027.8714963
GTATCGC1958.265042E-626.2226831
CGCTCTG26200.026.102475
GTATAGA14150.024.779841
GTGTTAG30450.023.9897881
GTACTAA8900.023.802451
GTGTAAG23000.023.5026911
GTGTATA26100.023.230111
TTGCTCT60550.023.0646514
GTATTAT15850.023.0438181
GTGTAAC17050.022.7072851
GTACTAC7750.022.6215951
GTATAGG12600.022.6103741
GTATTAG15950.022.4413551
Produced by FastQC (version 0.11.5)