FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4642844
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG20150.042.514278
GTCTCGC19400.039.8845561
CTGTCGC26350.034.696529
GTCTTGC44250.031.1780661
TCGCTCT21400.029.9397684
TCTCGCT24500.027.6605722
GTATTAG17050.026.1159651
GTGTAAC14800.025.6473351
GTATAGA12200.025.1298641
GTCTTAC16700.024.0406861
CGCTCTG25500.023.7143555
GTGTAGG26600.023.6002961
GTGTTAG32350.023.4671151
CTCGCTC26200.023.389293
GTCTCAC53450.023.3533421
GTGTAAA25950.022.7849621
GTATTAA13150.022.7592931
GGGTTTC78300.022.6540151
TACACTG26300.022.4455535
GTATTAC11900.022.0829051
Produced by FastQC (version 0.11.5)