FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4642844
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG19250.039.270688
GTCTCGC20100.035.701751
GTCTTGC40450.034.5759661
TCGCTCT23350.030.5222194
CTGTCGC26500.030.156929
TCTCGCT25000.028.2426072
CTCGCTC25000.027.643883
GTCTTAC18400.026.2654951
GTGTTAG32350.025.3514731
CGCTCTG25400.024.9411645
GTATTAG14400.023.899831
GTATACG3702.3101165E-1023.7486551
GTCTCAC51250.023.717761
GGGTTTC76950.023.5994871
GTTAGCC25100.023.23163
TTGCTCT58450.022.7853134
GTTTCAC67600.022.1505473
GTGTTAC19100.021.8524911
GTATAAT12300.021.4317111
TGTTAGC30000.021.3740832
Produced by FastQC (version 0.11.5)