FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4473439
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC20700.039.5114751
TCTGTCG20050.038.7771538
TCGCTCT22750.035.757214
GTCTTGC41600.034.7574121
CTGTCGC25750.032.7095959
TCTCGCT24450.031.8415582
CTCGCTC25000.029.9876733
CGCTCTG25750.029.3547655
GTCTTAC17800.025.4359071
GTACTAT9450.024.728271
GGTATAC9250.023.6840021
GTACAGG24800.023.262211
GTCTCAC52000.022.7503051
TTGCTCT58050.022.5702154
CTGTTGC48950.022.501189
GTATTAC10450.022.3619271
GGGTTTC77850.022.2313211
GTATAAC8400.021.733831
GTATAGA13150.021.65781
GTTTCAC71250.021.5498473
Produced by FastQC (version 0.11.5)