FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4473439
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG19850.039.1690648
GTCTTGC42450.036.2631
GTCTCGC21450.035.540881
CTGTCGC25750.033.5493359
TCGCTCT24450.030.0321674
TCTCGCT27600.027.6797682
CGCTCTG27450.025.96325
GTATTAG13850.025.9339371
GTCTTAC18150.024.6362921
CTCGCTC29750.024.197943
TTGCTCT56550.024.1872434
GTATTAA12800.024.0525681
GTGTTAG31050.023.371991
GTACTAT9800.023.1877041
GTCTCAC53450.022.765781
GGTATAC9750.022.5547891
GTGTTAC19250.022.0861191
GGAGTGC106200.021.9627065
GGGTTTC72850.021.935581
GTATAGG12050.021.8996721
Produced by FastQC (version 0.11.5)