FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4071682
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16800.044.992158
GTCTCGC19250.037.5416761
GTCTTGC37900.035.6321261
TCGCTCT22100.034.202184
CTGTCGC23900.033.7347039
TCTCGCT22000.031.7828522
CTCGCTC23950.028.8941233
GTATCGC2354.0277882E-827.9565681
CGCTCTG24700.027.3960325
GTATTAG15450.026.9311921
GTATAAT11500.026.0252691
GGTATAC8100.025.2337721
GTCTCAC49850.024.89391
TTGCTCT50300.024.3297524
GTATTAA12650.024.2363911
GTATTAC9650.024.2064931
GTACTAT9800.023.091111
GTATTGC10000.022.629291
GTTTCAC60500.022.2807623
CTTGCTC52100.022.275733
Produced by FastQC (version 0.11.5)