FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4071682
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18100.036.9907238
GTCTCGC19100.034.9002231
GTCTTGC38600.032.8306161
TCGCTCT19450.032.20124
CTGTCGC23900.031.9298139
CTCGCTC21750.028.464683
GTCTTAC16000.028.3851991
GCGTAGT4050.027.1304171
CGCTCTG23500.026.0389525
GTCTCAC51900.025.5465011
GTATTAC9250.024.5493621
TCTCGCT25100.024.4027732
TTGCTCT53550.023.6605914
GGTATAC8050.023.6590731
GTGTTAC16450.022.2650831
ACTGTCG4557.2759576E-1222.15168
TAATACT13550.021.7829654
GTTTCAC62650.021.71743
CTTGCTC54450.021.6826843
GTATATA19400.021.5225321
Produced by FastQC (version 0.11.5)