FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4046836
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG18300.049.9572948
CTGTCGC23150.040.423949
GTCTCGC18350.040.1775441
TCGCTCT22050.033.9532854
GTCTTGC40400.033.0649451
TCTCGCT22400.031.5362192
CGCTCTG22700.030.4440485
CTCGCTC25500.028.8331153
GTATAAT12600.025.4906081
GTGTAAT17550.024.539911
GTATAGG10450.024.4483641
GTATAGA13250.024.2401261
TTGCTCT57350.023.8493864
CTTGCTC54250.023.7839983
GTCTTAC16650.023.2359121
TCTTGCT61900.022.0091232
GTGTAGA20650.021.9164261
GTTTCAC63600.021.7608433
GTCTCAC50450.021.7034191
GTTCTAC12450.021.693541
Produced by FastQC (version 0.11.5)