FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768753
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15350.045.4981358
GTCTCGC15350.039.8827251
CTGTCGC20150.035.0171859
TCGCTCT18250.033.534074
TCTCGCT18700.032.72712
GTCTTGC36550.032.316981
GTGTTAC15300.029.1859971
CTCGCTC21700.027.207233
GTATAAT11200.026.365751
CGCTCTG20700.026.0868195
GTATTAG12150.024.3042281
TTGCTCT49700.023.0340844
GTCTCAC48050.022.9336051
TCTGTCA46850.022.5911318
GTACGCG1650.002708934521.8180663
GTACTAC6650.021.6612051
GTGTAAA22350.021.5909421
GGGTTTC64200.021.5397431
GTGTTAG27000.021.0735451
TACACTG21900.021.0409855
Produced by FastQC (version 0.11.5)