FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3768753
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16850.035.8929678
GTCTCGC16150.033.4563141
CTGTCGC21200.032.943219
GTCTTGC35300.031.2252621
TCGCTCT17850.027.8317744
GTATTAC8700.027.3265041
TCTCGCT20750.024.6369842
CTCGCTC20800.023.8844783
GTATAAC7150.023.1745411
GTCTCAC47200.022.5896281
GTATTAG11600.022.3580471
GTGTAGG23600.022.2843631
GTACTAA9100.022.1669521
GTATTGC9900.021.8310931
GAGTGCA94700.021.5922746
GTTAGCC20800.021.4614143
GGTATAC8450.021.3143791
GTATAAA13550.021.2671891
ACGTCGT1700.003221184221.176356
GGAGTGC86950.020.9498525
Produced by FastQC (version 0.11.5)