FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834152
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13000.047.6301048
TACACCG2057.2759576E-1238.6336065
GTCTCGC13700.037.3245431
CTGTCGC17900.034.9939239
GTCTTGC29150.032.860171
GTCGTAC950.004884697530.3244611
GCGTAAC1504.605216E-528.8082391
GTATTAG9400.028.3485321
TCGCTCT14800.028.2158224
TTGCTCT36850.027.1583734
TCTCGCT16450.027.13642
GTACGGA2705.980837E-926.6742941
GTATAAG8400.026.579031
GTATTAA8600.025.1234631
GTTTCAC42700.024.786543
GTCTTAC9100.024.5344871
GGGTTTC47950.024.0318981
GTGTTAG20400.023.6538241
CTCGCTC18450.023.4143073
CGCTCTG17700.023.1861155
Produced by FastQC (version 0.11.5)