FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988743
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13300.048.1915781
TCTGTCG12650.042.6868978
TCGCTCT14000.038.5706564
TCTCGCT15850.038.1569632
GTCTTGC29100.035.8845751
CTCGCTC15900.035.772873
CTGTCGC15850.035.431479
GTCTTAC11150.032.2944561
TTGCTCT37700.027.8827324
GTGTAAC9200.027.397631
GTATAAC5300.027.176091
GGTATAC6350.026.0847641
CGCTCTG18500.025.6859725
GTATTAG9650.025.3737371
CTTGCTC35850.025.3049343
GTTTCAC45000.024.7995053
GTACGGG1751.317165E-424.6852196
GTATACG2351.4118123E-624.5162981
GTGTTAC12450.024.2947671
GTGTAGG18100.024.27081
Produced by FastQC (version 0.11.5)