FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988743
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12400.041.2271468
GTCTCGC15150.037.562111
TCGCTCT14800.035.0275764
CTGTCGC16350.031.2670679
CTCGCTC16450.030.6377533
GTGTAAC7400.030.1762681
TCTCGCT18100.029.8352222
GTCTTGC29950.028.3805431
CGCTCTG17100.028.2109725
CGTTTCG1450.001277278524.828399
GTATTAC6000.024.0112231
GGTATAC6050.023.8127841
GGCTAGA19100.023.7597961
GTCTCAC35500.023.5377621
GTGTTAG18400.023.4892391
GTTTCAC43800.022.5201473
GGAGTGC70550.022.4525155
GTCCTAC10300.022.3793951
TTGCTCT37700.022.3451794
GTACTAG5850.022.1642071
Produced by FastQC (version 0.11.5)