FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2854903
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.048.188098
CTGTCGC17200.038.9295169
GTCTCGC13800.038.616781
TCGCTCT15000.036.959324
GTCTTGC27300.035.348061
TCTCGCT17100.031.5783672
GTGTAAC9150.031.4821
CTCGCTC16500.031.4176063
CGCTCTG17200.030.9761735
GTCTTAC10050.025.7964441
GTATAGA6750.025.605361
TTGCTCT36650.024.1632674
CTTGCTC35950.022.6310163
GGCTTAT10400.022.1584841
GTTTCAC40000.022.1395933
TTACACT13400.022.0294464
GGGTTTC43300.021.9537871
TACATCG2303.317657E-521.9126435
GTGCGAA2654.010715E-621.74041
GGAGTGC73700.021.6875255
Produced by FastQC (version 0.11.5)