FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3063201
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12850.041.4624568
GTCTCGC13500.038.4120061
GTCTTGC28300.036.13851
TCGCTCT14600.035.5063554
CTGTCGC17250.032.973459
TCTCGCT16600.031.6622092
GTGTTAC12650.029.6060891
CTCGCTC17200.029.3019163
GTCTTAC11650.027.8198531
GTATAAT7850.027.5245251
GTGTAAC10400.027.0084421
CGCTCTG17700.026.440315
GTATTAG9600.026.2582041
TTGCTCT38350.025.7206334
GTCTCAC35750.025.5856191
GTCTAAT8000.025.2078761
GGGTTTC50450.024.983031
GTATTAC7350.024.497451
GGTATAC5000.024.4876541
GTTTCAC46250.024.2850693
Produced by FastQC (version 0.11.5)