FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760206
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12550.041.3066378
GTCTCGC14150.037.1575431
TCGCTCT14650.035.8770144
GTCTTGC26200.032.9883461
CTGTCGC17150.032.3264249
TCTCGCT17550.030.3588772
GTATAAT6450.029.0331551
GTGTTAC11900.027.8414251
TACACTG15600.027.230685
CGCTCTG17300.027.0519355
CTCGCTC18350.026.681113
GTCTCAC33300.026.6036641
GTCTTAC10650.026.3751891
GTTACAC7250.025.8206043
GGTATAC5200.024.9315781
GTGTAAC7800.024.9315781
GCGTTAC1801.5906576E-424.0081841
GTATTAG9400.023.7527791
GTGTTAG18900.023.2460211
GGGTTTC44600.023.0930751
Produced by FastQC (version 0.11.5)