FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2760206
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11800.044.542028
GTCTCGC13200.042.582141
CTGTCGC16550.032.19319
TCGCTCT16450.031.5134264
TCTCGCT17450.030.1206512
GTCTTGC25200.028.3101061
GTATTAA7000.027.7953761
CTCGCTC18200.027.692093
CGCTCTG17850.026.6216375
GTATAAG8200.025.4853731
GTCTCAC34250.025.248031
GTGTTAG18100.025.0823841
GTGTTAC11550.024.9565681
GTCTTAC10400.024.944571
TGTACCG1801.5944267E-423.999815
TTGCTCT34000.023.929224
CTAACGT2151.9700992E-523.4416734
GGTATAC4950.023.2927971
GTCTACG1550.001874191723.2458341
CTAGTAC4053.45608E-1123.110933
Produced by FastQC (version 0.11.5)