FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927104
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12300.040.9744268
GTCTCGC12950.033.370081
GTCTTGC28750.032.567261
CTGTCGC16650.031.9990739
GCGTAAA1753.9767892E-628.8095021
TCGCTCT14450.028.400564
TCTCGCT14800.027.7289262
GTATTAT8500.026.2674871
GTGTAAC8100.025.7862821
GTGTAAA15450.025.6395241
GTATTAG9550.024.8877891
TACACCG2651.4739999E-724.4521225
GTATAAA11600.024.2148841
TTGCTCT38300.023.8739834
GTACAAG11000.023.5714111
GTCTCAC34150.023.4103571
GTGTTAC11400.023.3761311
TACACTG17850.022.990935
CTCGCTC18200.022.944393
ACCGTGT10250.022.4773988
Produced by FastQC (version 0.11.5)