FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2927104
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13000.048.741238
GTCTCGC13450.041.2428131
TCGCTCT14500.038.2360044
GTCTTGC26850.037.294971
CTGTCGC19250.034.03839
TCTCGCT16700.032.767212
CTCGCTC16450.031.9515463
CGCTCTG17300.029.9665095
GTATTAG8650.026.651011
GTGTAAA14700.026.4640451
TTGCTCT36600.025.3780574
GTCTCAC34650.025.3650911
ATACCGA1500.001556070424.0009566
GTGTTAG20350.023.7186641
GTTTCAC43250.023.6394393
TCTTGCT40150.022.7751122
GTATAAC4750.022.7497941
GTTAGCC15550.022.2252043
CTTGCTC37350.022.1687983
GGGTTTC48750.022.1664661
Produced by FastQC (version 0.11.5)