FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923265
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT14700.045.5502364
TCTGTCG13250.045.1011128
GTCTCGC14850.044.1340371
TCTCGCT16250.038.103962
CTCGCTC16400.036.8774153
CTGTCGC16850.035.037979
GTCTTGC27000.034.4100041
CGCTCTG18800.033.7015465
GTTTCAC40050.026.9658283
GTATTAG9300.026.3302351
GTGTTAC12850.025.7818131
GGTATAC5350.025.5775281
TCTCGAT11650.025.3386217
GTATAGA7800.024.9303251
GGGTTTC46350.024.7062111
TCGTTCG1500.001556586423.9995864
TTGCTCT36650.023.1810474
GTGTTAG20700.022.9631961
CTCGATC11950.022.8950028
GTCTCGA28050.022.5878476
Produced by FastQC (version 0.11.5)