FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923265
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15300.048.503731
TCGCTCT16250.044.307654
GTCTTGC25200.041.7428051
TCTCGCT18350.038.8453142
TCTGTCG13400.038.1492168
CTCGCTC18950.036.8542183
CGCTCTG18100.035.403285
CTGTCGC17700.030.9152269
GTATTAA7600.029.38851
GGTATAC4750.028.819691
GTCTTAC10000.028.819691
GTTTCAC40850.027.671473
TTGCTCT34950.026.3690744
GTCTCAC33450.026.2780441
GTGTAAC8300.026.041891
GGGTTTC45100.025.2411921
CTTGCTC37300.025.093383
TCCGTAC2301.1731645E-625.0430283
GTGTTAG20850.024.880311
TCTGTCA38500.024.8727058
Produced by FastQC (version 0.11.5)