FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2666145
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13000.043.761641
TCTGTCG12900.041.860078
GTCTTGC25350.039.2022481
TCGCTCT14050.036.3839954
TCTCGCT15250.036.3537562
CTGTCGC17250.035.4779249
CGCTCTG15550.032.411275
CTCGCTC16800.030.428283
GGTATAC5450.029.0693951
GTATTAC6100.028.3329141
TTGCTCT34950.026.5748564
GTATAAA9600.026.2546751
GTATTAA7500.025.9246141
GTTTCAC40750.025.4427013
CTTGCTC32550.025.2163493
GCGTAGT3151.1204975E-925.1473351
GGGTTTC42000.025.0330281
GTCTTAC10100.024.241941
GTTAGCC13450.024.088993
GTGTTAG18300.024.0042721
Produced by FastQC (version 0.11.5)