FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589577
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC25050.039.1147541
TCTGTCG12900.037.954238
GTCTCGC11400.035.390981
CTGTCGC16850.032.902729
TCGCTCT13300.031.399114
TCTCGCT13400.028.4787182
GTACTAT5350.028.2797091
GTCGTAA1555.7444508E-527.8887441
GTGTTAG19050.027.2299581
CTCGCTC13800.027.129923
GGGTAAT7000.026.7599161
GTCTTAC10650.026.3830151
GTTAGCC14200.025.8586643
TTGCTCT36200.025.657964
GTCTAGC10700.024.9130761
GGGTTTC40800.024.8982241
CGCTCTG16600.024.7233775
GTTTCAC36300.024.3962293
TCTCGAT10950.024.3292437
TGTTAGC17000.023.7185692
Produced by FastQC (version 0.11.5)