FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466882
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC12100.036.6304781
GTCTCGC6550.034.3889161
TCGCTCT6350.034.0134
TCTGTCG5750.031.301828
GTACCGG950.00467714330.5938781
GGTCCGA1000.006286747828.7976766
TTGCTCT17200.028.0442494
GAACCGT1555.7709512E-527.8687176
CTGTCGC8550.026.9488689
CTCGCTC7800.026.7670673
GTATTAT4650.026.5640371
GTACTCG1658.295467E-526.4219841
GTGTTAC5300.026.0480881
TCTCGCT8350.025.8661772
GTGTAAT5900.025.8621941
GTATAGA3950.025.7530731
GGTATAC2859.673386E-925.4948961
GAGTGCA35700.024.804726
GCGTAGT1500.001475479724.2201521
GGCTACG1500.001475479724.2201521
Produced by FastQC (version 0.11.5)