FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459635
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG4500.052.794868
GTCTCGC4950.048.4316641
TCGCTCT5400.041.329314
ACCGACG700.001093922441.138858
GGTTACG951.0168762E-438.2355271
TCTCGCT6600.037.110182
CTGTCGC7400.035.0236179
GCGTAGT1707.6883225E-834.1870571
CGCTCTG6750.033.063455
CTCGCTC6900.032.3646353
GTACTCG1000.006015315729.0591
GTCTTGC13200.028.618711
ACCGTGT4850.028.2034448
GTCTCAC16200.027.3549231
GTCTAGA6450.027.0316261
GTGTTAG9900.026.4172731
GTTTCAC20550.025.9404813
CTGTTGC15900.025.3560269
GGTATAC3201.2132659E-924.9725781
CTCGATC5500.024.8703088
Produced by FastQC (version 0.11.5)