FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459635
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6200.046.446898
GTCTCGC7800.041.881451
TCGCTCT7500.038.39614
GTGTACG1655.9513695E-835.1973421
GTCTTGC13000.035.1804161
CTCGCTC8350.034.487513
GTACACG1551.4507714E-632.7846151
CTGTCGC8150.030.9181619
GTATTAC3550.030.6737351
GTATTCG950.004697956630.5661091
TTAGCGC950.004893196330.3127083
TTAGACG1204.2294007E-429.9969524
TCTCGCT9400.029.8693072
CGCTCTG8200.029.8506265
GCGTAGG1753.7380414E-629.0378061
GTACAGG7400.028.4492021
TCTGTCA17850.028.2324268
GGTAACG1306.4351567E-427.9209671
GTGTTAG8450.027.4914131
GTACTAG2405.208858E-827.2229421
Produced by FastQC (version 0.11.5)