FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314027
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5950.037.5066688
TCGCTCT6150.037.4603354
GTCTTAC4200.034.6095241
GTCTTGC12400.033.409361
GCGTAGT1352.1066704E-532.3022231
GTCTCGC5550.030.119641
TAAGACG1204.228693E-429.9975344
GTATTAT4650.029.6972061
CTGTCGC7600.029.3637759
CGCTCTG6800.028.5858885
GTATAAG3453.6379788E-1227.386671
GTGTAAG5850.027.332651
CTCGCTC6600.027.2922973
GTCCTAT3254.9112714E-1126.8356931
GTATAAC3005.711627E-1026.6493361
TTGCTCT17150.026.4468064
TCTCGCT6700.025.8095512
CCCGTGT3451.1823431E-1025.0395158
TTACTCT6050.024.9896814
GTCTCAC14850.024.960811
Produced by FastQC (version 0.11.5)