FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314027
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC5750.042.9761431
TCTGTCG6900.039.646018
TCGCTCT6950.036.2547384
CTGTCGC8100.034.6652379
CTCGCTC7500.034.5559463
TACGGAT850.002839090333.8770877
TCTCGCT7750.032.5123142
GTCTTGC12200.032.169941
GTATACG950.00467074230.6022071
ATACCCG950.004893397430.3122314
CGCACTT1204.2295633E-429.996485
GTATAAC2451.9208528E-929.6654031
TACACTG6600.029.451095
CGCTCTG7750.028.7966215
GGCGTAA1306.3905516E-427.953941
GTCTCAC15000.026.1648861
GTTCTAC3657.2759576E-1225.8861121
TTATACT4001.8189894E-1225.1970444
CAACGCT1751.3174863E-424.6828174
TTCACCG2951.5397745E-824.4039175
Produced by FastQC (version 0.11.5)