FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314365
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6450.058.0410848
GTCTCGC5650.052.764791
CTGTCGC7900.045.5653579
TCGCTCT7200.043.9992334
TCTCGCT8050.036.7008862
CTCGCTC8550.035.397433
GTCTTGC11800.033.89141
GTACGGG900.003577005132.3166471
CGCTCTG8700.032.27535
GTGTAAC4550.031.9615171
AGCGGTA950.004892827530.3129547
TTACCCG1000.006284571328.7994984
CCCGATA1000.006286907528.7973048
GTGTTAC4800.028.7820131
GGTATAC3052.1827873E-1128.6081771
GTATTAC2802.5465852E-1028.5656071
GTTTCAC17550.027.5097263
GACTGCG2705.999027E-926.6641737
GTCTTAC4650.026.5830461
GGGTTTC20650.026.4088821
Produced by FastQC (version 0.11.5)