FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272873
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC5350.051.679311
TCGCTCT6400.041.6209684
TCTGTCG6400.041.6193358
CTCGCTC7050.038.8261073
GCGTTAT750.001535880638.3947759
GTATTAG4300.037.225551
GTACGGA1458.461411E-735.125051
TCTCGCT7000.034.987282
CTGTCGC7950.034.4104089
GCGACTT850.002838788633.877749
GGATATA3300.033.0722851
CGCTCTG7600.031.2601325
GTCTTGC12450.028.0516741
GTATTAC2354.1531166E-827.8651621
GTCTTAC4700.027.8651621
GTTAGCC6550.027.4933743
GGGTTTC20350.026.8153691
GTATAGG3657.2759576E-1225.9141751
AGGTCGT1400.001041624225.7107856
GTCCTAC4600.025.3074871
Produced by FastQC (version 0.11.5)