FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272873
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5750.050.0830888
GTCTTGC12150.035.3126261
GTGTTAG9000.034.7440341
TTCACCG3450.033.3874175
GTCTCGC5250.033.243461
CTGTCGC8700.032.274649
GTTTCAC17250.031.7180443
GTCTCAC14450.031.7049451
TCGCTCT6600.030.5418954
CGCTCTG6400.030.371465
GTTAGCC7450.029.956243
GTTTCGA1703.182351E-629.6436066
GGGTTTC19700.029.1618561
CCGGAGT2604.0126906E-927.6890813
GTGTTAT3950.027.6152171
GTAGTAC2354.6034984E-827.5712593
GTCTTAC4500.027.4720251
TAGCGCC1050.00797852827.4253754
ATGGCGT5600.026.9968556
GGTTTCA18950.026.9731122
Produced by FastQC (version 0.11.5)