FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248388
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG4700.055.141778
TCGCTCT5050.049.8965074
GTCTCGC5700.048.504711
GTATTAT3700.041.2945481
CTGTCGC6800.041.2887549
CGCTCTG6450.040.1824345
CTCGCTC5950.038.7533573
GTCTTGC9850.038.409821
TCTCGCT6900.037.594972
CCCGTCG900.003756026731.9958428
GTGTTAC5000.030.5579681
GTCTTAC4900.029.696761
GTGTAAA6950.029.3121991
GTATAGA3550.028.6929261
GTATTAA3153.274181E-1127.7169781
GTTACCC3800.026.5473253
GTGTAGT7700.026.4571151
GTCTCAC13600.026.2139421
GTCATAC3108.240022E-1025.8170221
GTATTAC2559.170253E-825.6789651
Produced by FastQC (version 0.11.5)