FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248388
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5600.037.2833488
TCGCTCT5600.037.2818534
GTCTTGC10450.036.8584331
GTCTCGC4500.033.9143941
GTGCGAC1102.402492E-433.03351
CTCGCTC5100.031.055613
CTGTCGC7450.029.9602329
CGCTCTG6200.029.0292555
CGTAAGA1050.00797796127.4257342
GTCTTAC4850.026.9716841
GTACGGG1357.97159E-426.9161871
GTGCTAC4750.026.0095371
GTATTGC2807.994458E-925.9548911
GTGTCGC2051.2668721E-524.8154091
GTCTAAC2951.3928002E-824.6351531
GTGTTAA4750.024.4795651
GTGTAGT6900.024.2245671
GTAAGAC5350.024.2217943
TACGGCT1500.001556869223.9975194
GTGTTAC5200.023.758711
Produced by FastQC (version 0.11.5)