FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726748
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG1459.2269875E-734.7395175
TCTGTCG7900.031.88128
GTATTAG4700.029.0962851
GTCTCGC7600.028.4111921
GTACTAG2853.5288394E-1027.779831
GTACCGC1306.769772E-427.6802963
GTCTTGC18300.027.5314271
GCGCTAC1358.4356597E-426.6574151
GTGTTAG11800.024.3983141
TCGCTCT7550.023.8307174
CTGTCGC10500.023.3014729
TTGCTCT22850.022.992214
TAATACT5050.022.8019854
GTGTTAC5700.022.7289521
GTGTTAA7300.022.6770611
TATCCCG1600.00227231422.4876335
GTATAGC3905.511538E-1022.1461621
GGGTAAC5650.021.6562021
TCTCGCT9050.021.473212
GTCTCAC20350.021.2211361
Produced by FastQC (version 0.11.5)