FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237511
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC4650.045.3796421
TCTGTCG5500.037.960278
GTCTTGC11450.037.494071
TCTCGCT5550.035.0579222
TCGCTCT5550.035.0253374
ACCCGAT850.002838659833.877987
GTACTAG1956.7520887E-933.5833471
GTCTTAC3850.030.239551
GGCTAGA7050.029.9312551
CTGTCGC7900.028.250669
CTCGCTC6700.027.964943
CGCTCTG6900.027.1291245
GTATAGA4300.025.3827611
GTGTTAA5050.024.4947831
TAATACT4151.8189894E-1224.2879834
GTATTAA3001.643275E-824.2546391
GTGCGGA2101.5122812E-524.2546391
CTAGAGT6300.024.0211683
TTGCTCT14850.023.7564284
GAGTGCA32150.023.511746
Produced by FastQC (version 0.11.5)