FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194430
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG4850.050.4685068
TCGCTCT5750.046.327124
GTCTCGC6250.044.2190061
CTGTCGC6600.042.5406349
GAACCGT1105.525724E-639.2682726
CGCTCTG7100.037.5184445
GTCTTGC11950.035.2992481
CTCGCTC6650.034.677533
TCTCGCT7150.033.2604142
GTACTAG2309.695214E-1031.6211411
GTCTTAC5050.030.2435861
GTCTCAC13850.029.9316351
GGTATAC2003.1666968E-729.0914521
CTTAACG1000.00625649128.8256952
TAGTACT2253.0162482E-828.797944
GTGCTAC4150.028.0399511
TTGCTCT16850.027.3452244
GTTCCGG1357.936281E-426.936531
TTCGCTC1658.772304E-526.205183
GTATTAG4350.025.0788361
Produced by FastQC (version 0.11.5)